KLF7 Transcription Factor Activity Assay

Measure the DNA-binding activity of Krüppel-like factor 7 (KLF7) in nuclear and cell lysates with this transcription factor activity assay from Assay Genie. The kit uses an absorbance-based microplate platform to quantify functional binding of active KLF7 to its consensus DNA response element, supporting research on this zinc-finger transcription factor across human, mouse, and rat samples. KLF7 contributes to neuronal differentiation, adipogenesis, and glucose homeostasis, and altered KLF7 activity has been reported in metabolic disease and several cancers, making quantitative measurement of its activation status relevant for neuroscience, metabolic, and oncology research programs.

The microplate workflow accommodates 96 determinations and is intended for research use only. Nuclear extracts or whole-cell lysates are added to wells pre-coated with a double-stranded oligonucleotide carrying the KLF7 binding motif, and captured protein is detected with an antibody-based chromogenic step read on a standard plate reader. Each kit ships with pre-coated microplate strips, nuclear extraction reagents, binding and wash buffers, a KLF7-specific detection antibody, substrate solution, and a stop reagent, providing a complete workflow for in vitro studies of KLF7 transcriptional regulation.