KIT (Phospho-Tyr703)Colorimetric Cell-Based ELISA Kit

From Assay Genie, the KIT (Phospho-Tyr703) Colorimetric Cell-Based ELISA Kit is a high-throughput, lysate-free assay designed to detect phosphorylation of the KIT protein at the tyrosine 703 residue in cultured cells. The cell-based format uses an indirect ELISA in which KIT (Phospho-Tyr703) is captured by a phospho-specific primary antibody, with an HRP-conjugated secondary antibody binding the Fc region of the primary antibody and catalyzing a colorimetric reaction measured at 450 nm.

The kit has a dynamic range of > 5000 Cells and is reactive with human and mouse samples. It can be used to measure the relative amounts of phosphorylated KIT in cultured cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on KIT phosphorylation. Because the assay is qualitative, two normalization methods are provided: a monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining step can be used to determine cell density, allowing absorbance values to be adjusted for plating differences.