KIF11/Eg5 Colorimetric Cell-Based ELISA Kit

Assay Genie offers a cell-based ELISA kit designed for the detection of KIF11/Eg5 protein in human adherent cell samples. The kit uses a colorimetric detection method read at 450 nm and operates in a 96-well microplate format, providing a high-throughput, lysate-free workflow for relative quantitation of KIF11/Eg5 in cultured cells. A dynamic range of > 5000 cells is supported, and reagents are stored at 4 °C with a stated stability of 6 months.

The assay follows an indirect ELISA format in which KIF11/Eg5-specific primary antibodies capture the target and HRP-conjugated anti-rabbit and anti-mouse IgG secondary antibodies are used for detection, producing a colorimetric signal upon substrate addition. Because the readout is qualitative, the kit includes a monoclonal anti-GAPDH antibody as an internal positive control and Crystal Violet solution for whole-cell staining to normalize for cell density. The kit can be used to compare KIF11/Eg5 expression across treatment conditions, including exposure to siRNA, chemical inhibitors, or activators.

KIF11 (also known as Eg5) is a kinesin-like motor protein involved in mitosis, contributing to chromosome positioning, centrosome separation, and the establishment of a bipolar mitotic spindle. The kit is categorized under the Cell Cycle research area, with human reactivity (UniProt ID: P52732; Gene ID: 3832).