Keratin 8 (Phospho-Ser432)Colorimetric Cell-Based ELISA Kit

Designed for the precise measurement of phosphorylated Keratin 8 (Phospho-Ser432) protein levels in cell lysates and tissue samples, this Cell-Based ELISA Kit from Assay Genie uses a colorimetric detection method at 450 nm. The assay operates with a dynamic range of > 5000 Cells and shows reactivity with Human, Mouse, and Rat samples. It is a lysate-free, high-throughput format suitable for adherent cells, allowing qualitative determination of Keratin 8 (Phospho-Ser432) via an indirect ELISA approach in which target-specific primary antibodies are detected with HRP-conjugated secondary antibodies.

Phosphorylation of Keratin 8 at Ser432 has been linked to cellular stress responses and to disease states such as inflammation, cancer, and liver diseases. The kit includes a monoclonal antibody specific for human GAPDH to serve as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density. Associated database information includes Gene ID: 3856, UniProt ID: P05787, and Gene Symbol: KRT8; K8 is described as a type II cytoskeletal keratin that typically dimerizes with keratin 18 to form intermediate filaments in simple single-layered epithelial cells.