Keratin 18 (Phospho-Ser52)Colorimetric Cell-Based ELISA Kit

This cell-based ELISA kit from Assay Genie is designed for the precise measurement of phospho-ser52 keratin 18 levels in cell lysates, offering high sensitivity and accuracy for research applications. The colorimetric assay is read at 450 nm and uses an indirect ELISA format in which Keratin 18 (Phospho-Ser52) is captured by phospho-specific primary antibodies while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, enabling a colorimetric reaction upon substrate addition.

The kit is reactive with human samples, has a dynamic range of > 5000 Cells, and is intended for use with adherent cells. It provides a lysate-free, high-throughput workflow that can detect Keratin 18 protein phosphorylation and expression profiles in cultured cells. The kit can be used to measure the relative amounts of phosphorylated Keratin 18 and to screen the effects of treatments, inhibitors (e.g., siRNA or chemicals), or activators on Keratin 18 phosphorylation. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density after HRP activity measurement.

Keratin 18 is a type I cytoskeletal keratin that, together with its filament partner keratin 8, is among the most commonly found members of the intermediate filament gene family and is expressed in single-layer epithelial tissues. Phosphorylation at serine 52 modulates its function, and dysregulation of keratin 18 phosphorylation has been linked to liver disease, cancer, and inflammatory disorders, making it a biomarker of interest in studies of disease pathogenesis and treatment strategies. The associated gene is KRT18 (Gene ID: 3875; UniProt ID: P05783), and the research area is the cell cycle.