KAM (Kanamycin) ELISA Kit

The Assay Genie KAM (Kanamycin) ELISA Kit provides quantitative detection of kanamycin residues in food, with a working calibration range of 0.5-40 ng/mL supporting compliance testing for veterinary antibiotic limits. Kanamycin is an aminoglycoside antimicrobial administered to livestock, and residual concentrations may persist in edible animal products. This competitive immunoassay is calibrated for meat, milk, and honey matrices, making it relevant for food safety laboratories, dairy processors, apiculture inspectors, and regulatory agencies monitoring drug residues.

The procedure uses a competitive ELISA format appropriate for small-molecule haptens, with colorimetric readout on a standard microplate reader. Each kit contains reagents for 96 determinations. Reagents should be stored refrigerated at 4 °C; repeated freeze-thaw cycles should be avoided to preserve antibody binding activity. Sample workflow involves homogenization, extraction into buffer, and dilution before loading the well plate.