JMY Colorimetric Cell-Based ELISA

A Cell-Based ELISA kit from Assay Genie provides a lysate-free, high-throughput and sensitive method for detecting JMY (Junction-mediating and -regulatory protein) directly in cultured cells. The kit is reactive with Human and Mouse samples and uses a colorimetric detection method read at 450 nm in a 96-Well Microplate format, with a stated dynamic range of > 5000 Cells. Storage conditions are 4°C for 6 Months.

The assay uses an indirect ELISA format in which JMY is captured by JMY-specific primary antibodies and detected with HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining step can be used to normalize results to cell density. The kit is suitable for measuring relative JMY levels in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on JMY expression.

JMY is described as a nuclear p53/TP53 cofactor and a cytoplasmic regulator of actin dynamics that activates the Arp2/3 complex, and the kit is listed for research in Epigenetics and Nuclear Signaling. It is intended for the qualitative determination of JMY protein expression profiles in adherent cells.