JAK2 (Phospho-Tyr931)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the detection of JAK2 phosphorylated at tyrosine 931 in cultured cells. The assay uses a colorimetric method with absorbance measured at 450 nm and is reactive with human, mouse, and rat samples. It is intended for use with adherent cell lines as a high-throughput, lysate-free alternative for studying JAK2 phosphorylation and expression.

The kit employs an indirect ELISA format in which phosphorylated JAK2 (Tyr931) is captured by target-specific primary antibodies, which are in turn bound by HRP-conjugated secondary antibodies. Upon substrate addition, the conjugated HRP catalyses a colorimetric reaction. For data normalisation, a monoclonal anti-GAPDH antibody is included as an internal positive control, and a Crystal Violet whole-cell staining step can be used to determine cell density so that absorbance values can be adjusted for plating differences. The kit has a quoted dynamic range of > 5000 cells.

Researchers can use the assay to quantify relative levels of phosphorylated JAK2 and to screen the effects of treatments, inhibitors (such as siRNA or chemical compounds), or activators on JAK2 phosphorylation, supporting studies of JAK2 signalling in health and disease, including myeloproliferative neoplasms and autoimmune disorders.