JAK2 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed to measure JAK2 protein levels in cultured cells using a colorimetric readout at 450 nm. The assay uses an indirect ELISA format in which JAK2 is captured by target-specific primary antibodies and detected with HRP-conjugated secondary antibodies, providing a lysate-free workflow suited to high-throughput use with adherent cells.

The kit is supplied on a 96-Well Microplate format with a stated dynamic range of > 5000 Cells and reactivity reported for Human, Mouse, and Rat samples. A monoclonal antibody against human GAPDH is included as an internal positive control for normalization, and Crystal Violet whole-cell staining is provided to determine cell density. Storage is recommended at 4°C with 6 months stability. Researchers can use the kit to investigate JAK2, a non-receptor tyrosine kinase involved in signal transduction pathways regulating cell growth, differentiation, and apoptosis.