JAK1 (Phospho-Tyr1022)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA kit enables qualitative detection of phosphorylated JAK1 (Janus kinase 1) at tyrosine 1022. The assay uses an indirect ELISA approach in which phospho-specific primary antibodies capture JAK1 (Phospho-Tyr1022), and HRP-conjugated secondary antibodies bound to the Fc region of the primary antibody catalyze a colorimetric reaction upon substrate addition. It is reactive with Human, Mouse and Rat samples and is intended for use with adherent cells.

As a Cell-Based ELISA, the kit is lysate-free and provides a high-throughput workflow for measuring relative JAK1 phosphorylation in cultured cells and for screening the effects of various treatments, inhibitors (such as siRNA or chemicals) and activators on JAK1 phosphorylation. Detection is by absorbance reading at 450 nm, with a stated dynamic range of > 5000 Cells. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density after the colorimetric measurement.

JAK1 is a widely expressed non-receptor tyrosine kinase involved in the interferon-alpha/beta and interferon-gamma signal transduction pathways, coupling cytokine ligand binding to tyrosine phosphorylation of STAT (signal transducers and activators of transcription) proteins. Phosphorylation of JAK1 at tyrosine 1022 is a key event in the JAK-STAT signaling pathway, which plays a critical role in cell growth, differentiation and immune response, with dysregulation of this pathway implicated in cancer, autoimmune disorders and inflammatory conditions.