ISL2 Colorimetric Cell-Based ELISA

Researchers studying the intracellular localization of ISL2 in tissue culture cells can rely on the ISL2 Colorimetric Cell-Based ELISA Kit from Assay Genie. The assay uses a colorimetric detection method measured at 450 nm and is reactive with Human, Mouse, and Rat samples, with a dynamic range of > 5000 Cells. ISL2 is a transcription factor involved in neuronal development and differentiation, making it a relevant target for studies of neurodevelopmental and neurodegenerative processes.

The kit employs a lysate-free, high-throughput indirect ELISA format that allows qualitative determination of ISL2 protein expression in cultured cells. Detection is achieved by capturing ISL2 with ISL2-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization, and a Crystal Violet whole-cell staining method may be used to determine cell density for further normalization.

Supplied in a 96-Well Microplate format designed for adherent cells, the kit is suitable for measuring relative ISL2 amounts in cultured cells and for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators. Storage is at 4°C with a stability of 6 months. The target protein is catalogued under UniProt ID Q96A47 and has a molecular weight of 39,768 Da.