IRS-1 (Phospho-Tyr896)Colorimetric Cell-Based ELISA Kit

Manufactured by Assay Genie, this Cell-Based ELISA Kit is designed for the precise detection of IRS-1 phosphorylated at tyrosine 896 directly in cultured cells. The kit employs a colorimetric detection method read at 450 nm and has been validated for use with Human, Mouse, and Rat samples, with adherent cells as the indicated sample type.

The assay is lysate-free and uses an indirect ELISA format in which IRS-1 (Phospho-Tyr896)-specific primary antibodies are paired with HRP-conjugated secondary antibodies. The HRP enzyme conjugated to the secondary antibody catalyses a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density and adjust for plating differences.

IRS-1 is a key adaptor protein in insulin receptor signalling, and phosphorylation at tyrosine 896 is a critical event in this pathway. The assay has a dynamic range of greater than 5000 cells and can be used to screen treatments, inhibitors (such as siRNA or chemicals), or activators that influence IRS-1 phosphorylation, supporting research into insulin resistance, type 2 diabetes, obesity, and other metabolic disorders.