IRF2 Transcription Factor Activity Assay

The IRF2 Transcription Factor Activity Assay from Assay Genie is a DNA-binding ELISA kit that quantifies active IRF2 protein in nuclear and cell lysates. IRF2 (Interferon Regulatory Factor 2) is a key regulator of type I interferon signaling, innate immunity, and has been characterized as a tumor suppressor in hematopoietic contexts.

Researchers apply this in vitro assay to measure DNA-binding competent IRF2 from human, mouse, and rat samples, supporting studies in cytokine response, viral immunity, and oncogenic signaling pathways. The procedure involves incubation of nuclear extract with plates coated with an IRF2 consensus oligonucleotide, followed by detection with a specific antibody.

Nuclear extracts may be prepared fresh or from previously frozen cell pellets; store kit components refrigerated at 4°C and protect from light to preserve antibody activity. This microplate format enables quantitative comparison of transcription factor activation across treatment conditions.