IRF-3 (Phospho-Ser396)Fluorometric Cell-Based ELISA Kit

From Assay Genie, this fluorometric cell-based phospho ELISA kit provides a convenient, lysate-free, high-throughput and sensitive method for monitoring IRF-3 (Phospho-Ser396) phosphorylation and expression in cultured cells. The kit is designed for measuring the relative amounts of phosphorylated IRF-3 and for screening the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on IRF-3 phosphorylation.

The assay uses an indirect ELISA format. Target protein is captured by IRF-3 (Phospho-Ser396)-specific primary antibodies, while Dye 1- and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody, enabling fluorometric detection. The kit supports a dynamic range of > 5000 Cells and is reactive with Human, Mouse, and Rat samples.

For data normalization, the kit includes a monoclonal antibody specific for human GAPDH as an internal positive control, along with an antibody against the non-phosphorylated counterpart of IRF-3 (Phospho-Ser396). Fluorescence is read at Ex/Em 651/667 (Dye 1) and 495/521 (Dye 2). The kit is stored at 4°C with 6 months stability. The target gene is IRF3 (Gene ID: 3661; UniProt ID: Q14653).