IRF-3 (Phospho-Ser385)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the accurate detection of phosphorylated IRF-3 at serine 385 in cell lysates, with reactivity to human samples. The kit employs a colorimetric detection method measured at 450 nm and uses an indirect ELISA format in which target-specific primary antibodies are bound by HRP-conjugated secondary antibodies, catalysing a colour reaction upon substrate addition.

Phosphorylation of IRF-3 at serine 385 is a key event in innate immune signalling, regulating activation and nuclear translocation of this transcription factor to drive type I interferon production. The assay is described as convenient, lysate-free, high-throughput and sensitive, suitable for measuring relative levels of phosphorylated IRF-3 in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on IRF-3 phosphorylation. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalisation, and a Crystal Violet whole-cell staining step can be used to determine cell density for further normalisation of absorbance values. The dynamic range is stated as > 5000 cells. The kit is intended for research in the immunology area.