IRAK1 (Phospho-Thr100)Fluorometric Cell-Based ELISA Kit

A Phospho Cell-Based ELISA Kit from Assay Genie designed for the fluorometric detection of IRAK1 phosphorylation at Thr100 in cultured cells. The assay is lysate-free, high-throughput and sensitive, allowing researchers to monitor IRAK1 protein phosphorylation and expression profile directly in cells. It is suitable for measuring relative amounts of phosphorylated IRAK1 and for screening the effects of various treatments, inhibitors such as siRNA or chemicals, and activators on IRAK1 phosphorylation.

The kit uses an indirect ELISA format in which IRAK1 (Phospho-Thr100) is captured by phospho-specific primary antibodies, with Dye 1-conjugated and Dye 2-conjugated secondary antibodies binding the Fc region of the primary antibody to enable fluorometric readout at Ex/Em 651/667 nm and 495/521 nm. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target RFU values, and an antibody against the non-phosphorylated counterpart of IRAK1 is provided so that RFU values obtained for non-phosphorylated IRAK1 can be used to normalize the RFU value for phosphorylated IRAK1.

Validated for Human, Mouse and Rat reactivity, the assay has a dynamic range of > 5000 Cells and is stored at 4 °C with a stated stability of 6 Months. The target is Phospho-IRAK1 (Thr100), NCBI Gene Symbol IRAK1 (Gene ID: 3654, UniProt ID: P51617, OMIM: 300283).