IRAK1 (Phospho-Thr100)Colorimetric Cell-Based ELISA Kit

Designed for the detection and quantification of IRAK1 phosphorylated at threonine 100 in cell lysates, this Cell-Based ELISA Kit from Assay Genie offers a high-throughput, lysate-free workflow for studying innate immune signaling. The kit uses a colorimetric detection method at 450 nm in an indirect ELISA format, in which phospho-specific primary antibodies capture the target and HRP-conjugated secondary antibodies generate the signal. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density and adjust for plating differences.

The assay is reactive with human, mouse and rat samples, with a stated dynamic range of > 5000 cells, and is intended for adherent cell samples. Researchers can use the kit to measure relative levels of phosphorylated IRAK1 in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on IRAK1 phosphorylation. Database references for the IRAK1 target include Gene ID 3654, UniProt ID P51617, OMIM 300283, and Unigene Hs.200716/Hs.522819.