IP3KC Colorimetric Cell-Based ELISA

From Assay Genie, this Cell-Based ELISA Kit provides a colorimetric, indirect ELISA workflow for profiling the relative amounts of IP3KC protein expression in cultured human cells. The assay uses IP3KC-specific primary antibodies paired with HRP-conjugated secondary antibodies and a substrate read at 450 nm, and is configured in a 96-well microplate format suited to adherent cells. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining may be applied after the colorimetric step to normalize readings to cell density.

The target, IP3KC, is the protein product of UniProt accession Q96DU7 (Gene ID 80271; gene symbol ITPKC), a member of the Ins(1,4,5)P(3) 3-kinase family that catalyses inositol trisphosphate 3-kinase activity and localises to the cytosol and nucleoplasm. Variants in ITPKC have been associated with Kawasaki disease, making the kit relevant to research on IP3 signaling in cell growth, differentiation, apoptosis and in related metabolic, neurological and cancer contexts.

Reactivity is stated for human samples, with a reported dynamic range of > 5000 Cells and a detection range of at least 5000 cells/well. The kit is stored at 4°C with a stated shelf stability of 6 Months.