Interferon-gamma Receptor alpha Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed for the qualitative detection of Interferon-gamma Receptor alpha protein expression in cultured cells, offering high sensitivity and specificity for accurate and reliable results. The kit provides a lysate-free, high-throughput workflow that uses an indirect ELISA format, where target-specific primary antibodies capture Interferon-gamma Receptor alpha and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition.

The assay is intended for adherent cells, with reactivity confirmed for Human, Mouse, and Rat samples, and a dynamic range of > 5000 Cells. Detection is performed colorimetrically at 450 nm, in a 96-Well Microplate format, with recommended storage at 4°C for 6 Months.

For data normalization, a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control. Following colorimetric measurement of HRP activity, the Crystal Violet whole-cell staining method may be used to determine cell density, allowing absorbance values to be normalized to cell amounts and plating differences to be adjusted. The kit supports measurement of relative Interferon-gamma Receptor alpha amounts in cultured cells and can be used to screen the effects of treatments, inhibitors such as siRNA or chemicals, or activators on target expression.