IL-3Rbeta (Phospho-Tyr593)Colorimetric Cell-Based ELISA Kit

Designed for the detection of phosphorylated IL-3Rbeta protein in cell lysates, this Colorimetric Cell-Based ELISA Kit from Assay Genie enables researchers to measure IL-3Rbeta activation in cells with high sensitivity and accuracy. The kit targets IL-3Rbeta phosphorylated at Tyr593, a critical event in the receptor's signaling pathway that regulates cell growth, differentiation, and survival. It is validated for use with Human, Mouse, and Rat samples and is suited to adherent cells.

The assay uses an indirect ELISA format in which IL-3Rbeta (Phospho-Tyr593) is captured by a target-specific primary antibody and detected using an HRP-conjugated secondary antibody that catalyses a colorimetric reaction measured at 450 nm. A monoclonal GAPDH antibody is included as an internal positive control, and a Crystal Violet whole-cell staining method can be used to normalise results to cell density. The dynamic range of the assay is > 5000 Cells.

This lysate-free, high-throughput kit is suitable for measuring the relative amounts of phosphorylated IL-3Rbeta in cultured cells and for screening the effects of various treatments, inhibitors, or activators on IL-3Rbeta phosphorylation, making it relevant to studies in immune responses, cancer development, and inflammatory diseases. The associated gene is CSF2RB (Gene ID: 1439, UniProt ID: P32927).