IL-3Rbeta Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed for the precise measurement of IL-3 receptor beta (IL-3Rbeta) levels in cell samples, providing a sensitive tool for research into immune regulation and hematopoiesis. The kit uses a colorimetric detection method read at 450 nm and is intended for adherent cells, with reactivity established for Human, Mouse and Rat samples.

The assay is lysate-free and high-throughput, using an indirect ELISA format in which IL-3Rbeta is captured by target-specific primary antibodies and detected via HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining step can be used to determine cell density for further normalization. The kit can be used both for measuring relative amounts of IL-3Rbeta in cultured cells and for screening the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on IL-3Rbeta expression.

Stated performance specifications include a dynamic range of > 5000 Cells, a microplate format, and storage stability at 4°C for 6 months. The target corresponds to the CSF2RB gene (Gene ID 1439; UniProt ID P32927), and dysregulation of IL-3Rbeta has been implicated in disease processes including leukemia and autoimmune disorders.