IL-2Rbeta Colorimetric Cell-Based ELISA Kit

This Cell-Based ELISA Kit from Assay Genie is designed for the quantitative measurement of IL-2Rbeta (interleukin-2 receptor subunit beta) levels in cell culture samples. The assay is supplied in a 96-Well Microplate format and uses a colorimetric detection method read at 450 nm. It is reactive with Human, Mouse, and Rat samples and has a stated dynamic range of > 5000 Cells.

The kit is described as a convenient, lysate-free, high-throughput and sensitive assay that detects the IL-2Rbeta protein expression profile in cells. Qualitative determination of IL-2Rbeta concentration is achieved by an indirect ELISA format, in which IL-2Rbeta is captured by IL-2Rbeta-specific primary antibodies and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. The kit can be used to measure the relative amounts of IL-2Rbeta in cultured cells and to screen for the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on IL-2Rbeta. The indicated sample type is adherent cells.

Two normalisation methods are included: a monoclonal antibody specific for human GAPDH serves as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining method is provided to determine cell density so that absorbance values can be normalised to cell amounts. Recommended storage is 4°C with a stability of 6 Months. Database identifiers associated with the IL2RB target include Gene ID: 3560 and UniProt ID: P14784.