IL-13R/CD213alpha1 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the sensitive detection of IL-13R (also known as CD213alpha1) protein expression in cultured cells. Detection is achieved by a colorimetric method at 450 nm using an indirect ELISA format, in which IL-13R/CD213alpha1-specific primary antibodies are bound by HRP-conjugated secondary antibodies that catalyse a colour reaction upon substrate addition. The assay is described as lysate-free and high-throughput, with a procedure suited to adherent cell samples.

Because the determination is qualitative, the kit provides two normalisation routes: an included monoclonal anti-GAPDH antibody serves as an internal positive control for normalising target absorbance values, and Crystal Violet whole-cell staining can be used to assess cell density so that absorbance values can be adjusted to cell amounts. The kit can be used both for measuring relative IL-13R/CD213alpha1 levels in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators.

Reported reactivity covers Human, Mouse, and Rat samples. The stated dynamic range is > 5000 Cells. The kit is provided in a 96-Well Microplate format with recommended storage at 4°C for 6 Months. Database identifiers listed in the source include Gene ID: 3597 and UniProt ID: P78552.