IL-10Ralpha Colorimetric Cell-Based ELISA Kit

Designed for the precise measurement of IL-10Ralpha levels in cell lysates and tissue homogenates, this cell-based ELISA kit from Assay Genie provides a lysate-free, high-throughput workflow for profiling IL-10Ralpha protein expression in cultured cells. The qualitative, indirect ELISA format uses IL-10Ralpha-specific primary antibodies with HRP-conjugated secondary antibodies to catalyze a colorimetric reaction, and is suitable for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on IL-10Ralpha.

Detection is performed by colorimetric measurement at 450 nm with a dynamic range of > 5000 Cells, and the assay shows human reactivity. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density. The kit is intended for use with adherent cells and is stored at 4°C with a stability of 6 Months. IL-10Ralpha (gene symbol IL10RA; UniProt Q13651; Gene ID 3587) is a receptor for interleukin 10 that mediates the immunosuppressive signal of IL-10, with dysregulation linked to autoimmune diseases, infections, and inflammatory disorders.