IKK-gamma (Phospho-Ser85)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed for the accurate detection of IKK-gamma phosphorylation at serine 85 in cell lysates or tissue samples. The kit uses an indirect ELISA format in which IKK-gamma (Phospho-Ser85) is captured by phospho-specific primary antibodies, with HRP-conjugated secondary antibodies binding the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. Readout is performed at 450 nm, and the assay is reported to offer high sensitivity and reproducibility for studying IKK-gamma phosphorylation in cultured cells.

The kit is configured as a cell-based, lysate-free, high-throughput assay in a 96-well microplate format, suitable for adherent cell samples and for measuring the relative amounts of phosphorylated IKK-gamma, as well as screening the effects of various treatments, inhibitors (e.g. siRNA or chemicals), or activators on IKK-gamma phosphorylation. It is reactive with human samples and has a dynamic range of > 5000 Cells. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing the target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density and adjust for plating differences.

IKK-gamma, also known as inhibitor of nuclear factor kappa-B kinase subunit gamma (gene symbol IKBKG; UniProt Q9Y6K9), is a regulatory subunit of the IKK core complex and a critical regulator of the NF-kappa B signaling pathway, which plays key roles in inflammation, immune response, and cell survival. Phosphorylation at serine 85 can modulate IKK-gamma activity, with downstream effects on gene expression and cellular processes relevant to inflammation, autoimmune disorders, and cancer. The kit falls within the Epigenetics and Nuclear Signaling research area and is intended as a tool for studying IKK-gamma signaling and identifying potential therapeutic targets.