IKK-gamma (Phospho-Ser31)Colorimetric Cell-Based ELISA Kit

Designed for the precise measurement of IKK-gamma phosphorylation at Ser31 in cell samples, this colorimetric cell-based ELISA kit from Assay Genie provides a sensitive, lysate-free and high-throughput workflow. The assay uses an indirect ELISA format: phospho-specific primary antibodies capture the target, while HRP-conjugated secondary antibodies catalyze a colorimetric reaction that is read at 450 nm. A monoclonal anti-GAPDH antibody is included as an internal positive control, and Crystal Violet whole-cell staining can be used to determine cell density and normalize absorbance values.

The kit is reactive with human samples and is suited to adherent cells. It is configured for a microplate workflow, with a dynamic range of > 5000 Cells per well. The intended research area is epigenetics and nuclear signaling, where IKK-gamma (gene symbol IKBKG; UniProt ID Q9Y6K9; also called NF-kappa-B essential modulator) is studied as a key regulator of the NF-κB pathway, with roles in inflammation, immunity and cell survival. Phosphorylation of IKK-gamma at Ser31 modulates its activity, making it a relevant target when investigating signaling mechanisms and potential therapeutic approaches in cancer and inflammatory disorders.