IKK-alpha (Phospho-Thr23)Colorimetric Cell-Based ELISA Kit

A cell-based ELISA kit from Assay Genie is designed for the qualitative detection of IKK-alpha phosphorylation at Thr23 in cultured cells. The kit uses a colorimetric detection method at 450 nm and an indirect ELISA format, in which the phosphorylated target is captured by phospho-specific primary antibodies and detected via HRP-conjugated secondary antibodies.

The assay is reactive with human, mouse, and rat samples and supports a dynamic range of > 5000 cells, making it suitable for adherent cell cultures. It can be used to measure relative levels of phosphorylated IKK-alpha as well as to screen the effects of treatments, siRNA, chemical inhibitors, or activators on IKK-alpha phosphorylation.

For normalization, a monoclonal anti-GAPDH antibody is included as an internal positive control, and a Crystal Violet whole-cell staining step is provided to account for cell density. The target corresponds to the CHUK gene (Gene ID: 1147; UniProt ID: O15111), with a calculated molecular weight of 84 kDa and an observed molecular weight of 85 kDa.