IkappaB-alpha Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed for the quantitative measurement of IκBα (IkappaB-alpha) levels in cell lysates and tissue homogenates, with reactivity against human, mouse and rat samples. It uses an indirect ELISA format in which IκBα is captured by IkappaB-alpha-specific primary antibodies and HRP-conjugated secondary antibodies catalyze a colorimetric reaction measured at 450 nm, allowing detection of the IkappaB-alpha protein expression profile in cultured cells in a convenient, lysate-free workflow suitable for adherent cells.

The kit is supplied in a 96-well microplate format with a dynamic range of > 5000 cells, supporting high-throughput work. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization, and the Crystal Violet whole-cell staining method can be used to determine cell density so that absorbance values can be adjusted for plating differences. It is intended for immunology research, where IκBα is a key regulator of the NF-κB signaling pathway and plays a role in inflammation, immune response and cell survival; the protein corresponds to UniProt ID P25963 (gene NFKBIA, molecular weight 35,609 Da) and is an NF-kappa-B inhibitor that traps REL dimers in the cytoplasm until phosphorylation promotes its ubiquitination and degradation, enabling RELA nuclear translocation.

Storage stability is 4 °C for 6 months.