IGFBP-3 Colorimetric Cell-Based ELISA Kit

This Colorimetric Cell-Based ELISA Kit from Assay Genie is designed for the quantitative measurement of IGFBP-3 (insulin-like growth factor binding protein 3) levels in cell culture supernatants, serum, and plasma samples. The assay is a convenient, lysate-free, high-throughput and sensitive format for detecting IGFBP-3 protein expression profile in cells, and can be used to measure relative amounts of IGFBP-3 in cultured cells as well as to screen for the effects that various treatments, inhibitors, or activators have on the target. IGFBP-3 plays a critical role in regulating the bioavailability of insulin-like growth factors (IGFs) and modulating their signaling pathways, and changes in IGFBP-3 levels have been implicated in cancer, diabetes, and growth disorders.

The kit reacts with Human and Mouse samples, uses a Colorimetric readout at 450 nm, has a dynamic range of > 5000 Cells, and is supplied as a 96-Well Microplate format with recommended storage at 4°C for 6 months. It is classified as an ELISA Kit of Cell-Based type, with Cell Death listed as a research area, and targets IGFBP-3 (gene symbol IGFBP3).

Qualitative determination of IGFBP-3 concentration is achieved by an indirect ELISA format in which IGFBP-3 is captured by IGFBP-3-specific primary antibodies and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalising target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density, allowing absorbance values to be normalised to cell amounts.