IFN-_ Signaling Pathway ELISA Sampler Kit

The IFN-γ Signaling Pathway ELISA Sampler Kit from Assay Genie is configured as four coordinated 48-well microplates, enabling parallel measurement of multiple cytokines and signaling mediators implicated in interferon-gamma-driven immunity. Each plate is pre-coated with capture antibodies specific to individual analytes such as IFN-γ, IL-12p70, IL-2, IP-10 (CXCL10), and TNF-α, supporting profiling of the cytokine cascade triggered by Th1-polarized responses in human serum specimens, cell culture supernatants, and tissue homogenates.

The kit is optimized for use with 100 µL sample volumes per well, with a standard curve range typically spanning 15.6 to 1000 pg/mL per analyte. Assay Genie provides lyophilized recombinant standards, biotinylated detection antibodies, streptavidin-HRP conjugate, and wash buffer to support reproducible results across the four-plate workflow. Unopened components should be stored refrigerated, with opened reagents aliquoted and frozen to limit freeze-thaw cycles.