ICAM-1 (Phospho-Tyr512)Colorimetric Cell-Based ELISA Kit

A cell-based immunoassay from Assay Genie designed to detect ICAM-1 phosphorylated at tyrosine 512 in cultured cells. Using an indirect format with colorimetric detection at 450 nm, the kit provides a lysate-free workflow in which HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition. Phosphorylated ICAM-1 is captured by phospho-specific primary antibodies, enabling qualitative measurement of ICAM-1 phosphorylation and expression profile in cells.

ICAM-1 is a cell surface glycoprotein with a central role in immune response and inflammation, and phosphorylation at tyrosine 512 is known to modulate its function. The kit is therefore suited to research on cell signaling, inflammatory disease, autoimmune disorders, and cancer. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining step allows results to be normalized to cell number, accounting for plating differences.

Validated for use with human cells, including adherent cell lines, with a dynamic range of > 5000 Cells. The kit can be applied to measuring relative amounts of phosphorylated ICAM-1 in cultured cells and to screening the effects of various treatments, inhibitors such as siRNA or chemicals, or activators on ICAM-1 phosphorylation. Associated database entries: Gene ID 3383, UniProt ID P05362, OMIM 147840.