Human ZWILCH ELISA Kit (HUFI08261)

ZWILCH (Zw10-interacting kinetochore protein) is a core component of the RZZ kinetochore complex, working alongside ROD and ZW10 to regulate mitotic checkpoint signaling and the spindle assembly checkpoint. Quantitative measurement of human ZWILCH protein supports research into chromosome segregation, kinetochore function, and the molecular basis of mitotic fidelity in dividing cells. Investigators studying primary microcephaly, where biallelic ZWILCH variants have been linked to severe congenital microcephaly and primordial dwarfism, also use ZWILCH quantification to examine protein expression in patient-derived cells and to explore genotype-phenotype relationships. The Assay Genie kit is supplied as a 96 Assays pack and is intended for research use only in in vitro applications, supporting workflow reproducibility across cancer cell biology, genome instability, and developmental biology laboratories.

Typical laboratory workflows include quantification of ZWILCH in cultured human cell lines following experimental manipulation of kinetochore or checkpoint components, comparison of expression across tumor and matched normal tissue specimens, and characterization of patient-derived lymphoblastoid cell lines carrying ZWILCH mutations. The 96 Assays configuration is suited to mid-throughput studies that require biological replicates across multiple conditions, with the immunoassay components organized for batch processing of standard curves and unknowns simultaneously. As a research-use-only reagent intended for in vitro measurements, the kit is appropriate for academic and translational research programs investigating mechanisms of chromosome mis-segregation, aneuploidy, and the cell division defects that underlie certain developmental disorders and cancer genome instability.