Human ZNF654/Zinc finger protein 654 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed to quantitatively measure human Zinc Finger Protein 654 (ZNF654) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit supports research into ZNF654, a zinc finger protein implicated in gene regulation, transcriptional processes, DNA binding, and protein-protein interactions, with relevance to cancer development, neurological disorders, and immune responses.

Reported linearity (n=5 per matrix) is 88-105% for serum, 92-101% for EDTA plasma, and 82-97% for heparin plasma. Recovery spans 86-102% in serum (average 94%), 89-102% in EDTA plasma (average 95%), and 89-105% in heparin plasma (average 99%). Reagents are stored at 2-8°C for up to 12 months.

The double-antibody sandwich workflow involves loading standards and samples on the pre-coated plate and incubating at 37°C for 90 minutes, followed by a biotin-labeled detection antibody for 60 minutes and HRP-Streptavidin (SABC) for 30 minutes, with TMB substrate development in the dark for 10-20 minutes. Absorbance is read at 450 nm after the stop solution. Associated with Uniprot Q8IZM8, the kit is categorized under Epigenetics and Nuclear Signaling research, and ZNF654 is also referenced by the synonym Melanoma-associated antigen.