Human ZNF483 ELISA Kit (HUFI05547)

ZNF483 is a KRAB-domain zinc finger transcription factor expressed across human tissues, where it engages sequence-specific DNA binding and contributes to chromatin-based regulatory programs. This Assay Genie ELISA kit enables quantitative measurement of ZNF483 protein in human research specimens, supporting studies of zinc finger protein biology, transcription factor dynamics, and gene regulatory network composition at the protein level.

Configured for 96 individual determinations, the assay uses matched antibody pairs together with a chromogenic substrate to generate an absorbance-based readout on standard plate readers. Each kit ships as a complete reagent set that includes pre-coated wells, calibrated reference standards, detection antibody, wash buffer, and stop reagent. Common applications include expression profiling across cell types, comparative analyses of transcription factor abundance, and investigations into regulatory protein turnover and chromatin-associated factor dynamics.

Sample input per well is held to a microliter-scale volume consistent with the plate-based format, and clarified cellular lysates as well as human serum or corresponding plasma preparations serve as the principal validated matrices. Unused reagents should be stored refrigerated to preserve activity, and the supplied standard curve provides quantitative coverage across the working range. The product is supplied for research use only and is not cleared for diagnostic or therapeutic decision-making.