Human ZNF277 ELISA Kit (HUFI05559)

ZNF277 (zinc finger protein 277) is a human C2H2-type zinc finger transcription factor encoded on chromosome 8q21, with documented roles in DNA damage response and immune signaling pathways. The Assay Genie kit provides a plate-based quantitative immunoassay for research laboratories studying zinc finger protein function, gene regulation, and cellular stress responses in human cell systems.

Each pack contains a pre-coated well plate, antibody vials, standard diluent, wash concentrate, substrate solution, stop reagent, and assay buffer. Researchers commonly prepare cell lysates or tissue homogenates as starting material, dilute unknowns alongside a standard curve, and read the final colorimetric signal on a standard microplate reader. The supplied reagents are sufficient for one full plate of samples and standards run in parallel.

Unopened kits should be stored refrigerated until first use, with working dilutions of standards and detection antibody prepared fresh on the day of the assay and remaining components returned to cold storage between runs. The kit is intended for research use only, supporting applications such as comparing ZNF277 expression between treated and untreated cell populations, profiling its induction following DNA-damaging agents, or screening patient-derived material in translational research programs.