Human ZNF259 ELISA Kit (HUFI08276)

ZNF259, also known as ZPR1, is a zinc finger protein that binds the intracellular domain of the epidermal growth factor receptor and participates in the survival motor neuron complex. Altered expression of this conserved signaling molecule has been reported in research on spinal muscular atrophy and in studies of receptor tyrosine kinase-driven proliferation, so quantitative measurement of human ZNF259 supports investigations into neurodegeneration models and EGFR-related cell signaling pathways.

Supplied by Assay Genie, this colorimetric ELISA kit arrives as a complete microplate-based assay configured to run up to 96 determinations in a single experiment. The format allows researchers to generate a full standard curve alongside unknown samples in one plate, simplifying batch comparison across biological replicates.

Intended applications

• Quantification of human ZNF259 in cell lysates and tissue homogenates prepared from cultured lines
• Comparative studies of ZNF259 expression in patient-derived versus control samples
• Screening of EGFR signaling or SMN-complex-associated research cohorts
• Use as a complementary read-out alongside western blot or PCR assays in pathway-focused projects

Kit components

• Pre-coated microplate with plate sealers
• Recombinant ZNF259 standard for curve generation
• Biotinylated detection antibody and streptavidin-HRP reagent
• TMB substrate, stop solution, wash buffer concentrate, and assay diluent
• Printed protocol with recommended standard curve range

Store unopened kit components at the temperature indicated on the box and protect the TMB substrate from light. Allow all reagents to equilibrate to room temperature before beginning the protocol, and keep the standard and detection antibody away from repeated freeze-thaw cycles to preserve activity. For research use only.