Human ZBTB44 ELISA Kit (HUFI05615)

The ZBTB44 protein is a zinc finger and BTB domain-containing transcription factor that can be quantified in human clinical specimens using this colorimetric immunoassay from Assay Genie. This in vitro research-use-only ELISA kit is supplied as a complete reagent set, with pre-coated wells arranged across a microplate that supports 96 individual determinations.

Each kit contains a recombinant standard for generating a calibration curve, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent, all packaged in clearly labeled vials. Laboratory workflows typically allocate wells for blanks, standards, and unknown samples run in duplicate, allowing researchers to interpolate concentrations from the standard curve.

The assay is intended for research into zinc finger transcription factor biology, immune signaling pathways, and chromatin regulation, where ZBTB44 expression patterns have been linked to downstream gene control events. Prepared clotted or anticoagulated whole-blood fractions are loaded directly into wells after dilution in the supplied assay buffer.

Researchers handling the kit should equilibrate reagents to room temperature before use and protect the substrate from light exposure to maintain activity. Following the included protocol, absorbance values are read on a standard plate reader and ZBTB44 concentrations are calculated against the standard curve.