Human ZAG/AZGP1 ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the precise and efficient measurement of zinc-alpha-2-glycoprotein (ZAG/AZGP1) levels in human samples. The colorimetric, double-antibody assay is reactive with human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. It has a stated sensitivity of 1.875 ng/mL and a measurable range of 3.13–200 ng/mL, with storage recommended at 2–8°C for 12 months.

Zinc-alpha-2-glycoprotein is described as a key protein involved in lipid mobilization, regulation of glucose metabolism, and immune response modulation, and ZAG dysregulation has been linked to conditions such as obesity, type 2 diabetes, and certain cancers, making the kit useful as a potential biomarker research tool. The standard assay procedure includes a primary incubation at 37°C for 90 minutes, a 60-minute biotin-labelled detection antibody step, 30 minutes of HRP-Streptavidin binding, TMB colour development in the dark for 10–20 minutes, and absorbance measurement at 450 nm after addition of stop solution. Reported linearity for serum (n=5) at 1:2, 1:4 and 1:8 dilutions is 85–104%, 92–97% and 89–105% respectively, while recovery averages are 95% for serum, 93% for EDTA plasma and 96% for heparin plasma.

The kit is associated with Uniprot P25311 and the gene names AZGP1 / ZAG / ZNGP1, and is positioned for cell biology research applications investigating the role of zinc-alpha-2-glycoprotein in health and disease.