Human YTHDF3 ELISA Kit (HUFI05614)

Quantitative measurement of the YTHDF3 m6A reader protein is enabled by this Assay Genie ELISA Kit. YTHDF3 (YTH N6-methyladenosine RNA binding protein F3) acts as a reader of m6A modifications on messenger RNA, contributing to mRNA degradation, translation regulation, and processing of viral transcripts. Researchers in epitranscriptomics, oncology, immunology, and RNA biology use this assay to monitor YTHDF3 expression across experimental conditions, disease models, and treatment comparisons.

The kit pairs a pre-coated capture antibody with a matched detection antibody and uses a chromogenic substrate to generate a measurable absorbance signal at the end of the protocol. Reagents are supplied for 96 individual determinations in a standard microplate workflow that integrates with common plate readers in core facilities. It is calibrated specifically against the human YTHDF3 target and does not cross-react with mouse or rat orthologs, supporting species-specific measurement in translational and clinical research studies.

Each shipment includes the coated plate, matched antibody pair, lyophilized standards, and buffers needed to complete the full protocol from sample addition through final absorbance reading. Intended for research use only, the kit fits applications in cancer biology, viral-host interaction studies, mechanistic work on m6A-mediated RNA regulation, and biomarker screening across patient cohorts.