Human YAP1 ELISA Kit (HUFI05626)

The Hippo signaling pathway governs contact inhibition, organ size, and tissue homeostasis, and dysregulation of its transcriptional coactivator YAP1 (Yes-Associated Protein 1) is implicated in tumor initiation, progression, and chemoresistance across multiple cancer types. Researchers studying hepatocellular carcinoma, breast cancer, and lung adenocarcinoma rely on quantitative measurement of endogenous YAP1 protein to correlate pathway activity with clinical outcomes and to evaluate candidate therapeutics targeting the Hippo cascade.

This quantitative immunoassay from Assay Genie is configured as a 96 Assays microplate format intended for research use only. The assay supports investigations into YAP1 nuclear translocation, mechanotransduction responses to substrate stiffness, and crosstalk with TEAD transcription factors in cell culture models. Compatible workflows include parallel western blot validation of downstream targets such as CTGF and CYR61, alongside immunohistochemistry to confirm tissue-level expression patterns.

Each kit is shipped frozen on cold packs and should be stored refrigerated upon arrival to preserve antibody conjugate activity. Bring all reagents to room temperature before use, and avoid repeated freeze-thaw cycles of the standard and detection antibody vials.