Human XIAP PharmaGenie ELISA Kit (SBRS1241)

The Human XIAP PharmaGenie ELISA Kit from Assay Genie supports quantitative measurement of X-linked Inhibitor of Apoptosis Protein (XIAP), an anti-apoptotic regulator that binds active caspases-3, -7, and -9 to modulate programmed cell death signaling. The immunoassay incorporates a matched pair of monoclonal antibodies directed against distinct epitopes of human XIAP, with a biotin-streptavidin-HRP detection scheme and TMB substrate development.

Each kit provides pre-coated microplate wells, lyophilized standard calibrators, biotinylated detection reagent, enzyme conjugate, wash buffer concentrate, and stop solution sufficient for 96 determinations. The product is labeled for research use only and is commonly applied in cancer biology studies, drug response investigations, and signaling pathway research where XIAP expression levels serve as a biomarker of apoptotic resistance.

Unopened kit components should be stored refrigerated in their original packaging; the microplate should remain in its sealed foil pouch with desiccant to protect from moisture. Reconstituted standards are best aliquoted into single-use vials and held frozen to limit repeated freeze-thaw cycles.

Researchers typically load 100 µL of prepared sample per well, with all incubations performed at room temperature according to the included protocol. The plate arrives pre-blocked, and standard calibrators should be reconstituted shortly before each run.