Human WNT10A ELISA Kit

A sandwich (double antibody) ELISA from Assay Genie is designed for the quantitative measurement of human WNT10A (Protein Wnt-10a) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

The assay uses a microplate pre-coated with a target-specific antibody. After incubation and washing, a biotinylated detection antibody binds the captured target. HRP-conjugated Streptavidin is added, followed by TMB substrate to generate a colorimetric reaction that turns yellow upon addition of stop solution. Optical density is read at 450 nm, with OD450 proportional to analyte concentration as determined from a standard curve. The kit provides a detection range of 0.156-10ng/ml and a sensitivity of 0.094ng/ml. A validated Bradford Reagent is included for total protein quantification to support sample normalization. For research use only.