Human Wnt-5b ELISA Kit

A Sandwich ELISA using a double-antibody configuration from Assay Genie, designed for the quantitative detection of human Wnt-5b. The assay employs a colorimetric readout and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Wnt-5b is a secreted signalling ligand of the Wnt family that acts through the frizzled receptor family and is implicated in cell fate commitment, differentiation, and developmental patterning; dysregulation of the Wnt pathway has been associated with cancer, osteoporosis, and neurodegenerative disorders, making accurate quantification of Wnt-5b valuable for metabolism-related research.

Manufacturer-reported performance characteristics include a sensitivity of 0.469 ng/mL and a working range of 0.781-50 ng/mL. Linearity at 1:2, 1:4, and 1:8 dilutions is stated as 99-105%, 87-99%, and 90-98% for serum (n=5); 83-98%, 83-101%, and 82-99% for EDTA plasma (n=5); and 81-100%, 81-95%, and 80-92% for heparin plasma (n=5). Recovery ranges are 85-102% for serum (average 94%), 86-100% for EDTA plasma (average 93%), and 85-105% for heparin plasma (average 94%). Reagents are stored at 2-8°C for 12 months. Synonyms include WNT5B and Protein Wnt-5b (UniProt Q9H1J7).