Human Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit

WNT3A is a secreted glycoprotein ligand of the canonical Wnt/β-catenin signaling cascade. It binds Frizzled receptors and LRP5/6 co-receptors to drive β-catenin–dependent transcription, governing embryonic axis specification, osteoblast differentiation, and the maintenance of pluripotent stem cells in vitro. Aberrant WNT3A activity is reported in colorectal, gastric, and hepatocellular carcinoma models, making the ligand a frequent target in oncology biomarker studies.

The Assay Genie kit is supplied as a 96-assay plate and is validated for tissue homogenates, cell lysates, and cell culture supernatants, with a 50 µL sample volume per well. WNT3A-conditioned medium produced from transfected lines is a standard reagent for keeping mouse embryonic stem cells and human iPSCs in the naive or ground state, so quantitative readouts of the ligand are useful for stem-cell biology and regenerative medicine workflows.

Unopened kit components should be stored refrigerated at 4 °C and protected from light; reconstituted standards and the biotinylated detection reagent are best aliquoted into single-use vials and held at -20 °C to limit freeze-thaw loss of activity.