Human WFDC1 ELISA Kit (HUFI05658)

WAP Four-Disulfide Core Domain 1, also referenced in earlier literature as PS20, is a secreted glycoprotein bearing a conserved whey acidic protein motif with reported serine protease inhibitory activity, and the Assay Genie Human WFDC1 ELISA Kit enables its quantitative measurement in human specimens for research use only in cancer biology, mucosal immunology, and inflammation research. Aberrant WFDC1 expression has been documented in prostate tumour stroma, breast carcinoma microenvironments, and in airway epithelial cells from asthmatic donors, supporting interest in the protein as a biomarker candidate across reproductive and respiratory biology studies. Each kit is supplied as a complete pack with pre-coated microplate strips, matched antibody reagents, calibrated standard diluent, chromogenic substrate, wash buffer, and stop solution, and researchers can apply the assay to cell-culture supernatant from stromal fibroblast lines, conditioned media, or tissue research samples alongside downstream western blot and PCR validation. Unopened components are kept refrigerated until first use, with reconstituted standards best reserved as single-use aliquots to limit freeze-thaw cycles of the working material. The pack supports 96 individual determinations, and the procedure follows an absorbance-based workflow readable on a standard laboratory microplate reader.