Human WDSUB1 (WD repeat, SAM and U-box domain-containing protein 1) ELISA KIT (HUFI04792)

From Assay Genie, this WDSUB1 kit offers researchers a quantitative immunoassay for measuring the WD repeat, SAM and U-box domain-containing protein 1 in human research specimens. The kit contains a pre-coated microplate, recombinant protein standard, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, TMB substrate, and stop solution, with reagents supplied to support the full plate run.

The protocol relies on an HRP-TMB reaction captured as absorbance values for standard curve interpolation. Store unopened kit components refrigerated and protected from light; reconstituted standards and working conjugate should be frozen at -20 °C for longer-term stability. Each plate accommodates 100 µL sample additions per well, with the complete workflow finishing in roughly 2.5 hours at room temperature.

WDSUB1 encodes a U-box-type E3 ubiquitin ligase implicated in protein quality control and substrate turnover through the ubiquitin-proteasome system. This ELISA Kit supports in vitro research into ligase biology, post-translational modification pathways, and related cell-signaling studies. The protocol is compatible with both manual pipetting workflows and automated plate-washer systems, and the kit is intended for research use only.