Human WDR81 ELISA Kit (HUFI05643)

This quantitative immunoassay from Assay Genie enables measurement of WD Repeat Domain 81 (WDR81) protein levels for research applications. WDR81 functions as a scaffolding component of the endosomal sorting machinery and participates in autophagy regulation through interactions with mTORC1 pathway components. Mutations disrupting WDR81 expression have been characterized in cerebellar ataxia with mental retardation (CAMRQ2), hydrocephalus, and microcephaly, so accurate protein quantification supports neurological disease research, lysosomal trafficking studies, and investigations of neurodevelopmental signaling.

The microplate-based kit ships with pre-coated wells, a set of recombinant standards, detection reagents, wash buffer concentrate, substrate solution, and stop reagent sufficient for routine screening experiments. Each vial of reconstituted reagent should remain refrigerated and protected from light; the kit is intended for research use only and not for clinical diagnostic use.

For laboratories characterizing WDR81 in human cell lines, patient-derived fibroblasts, or brain tissue lysates, this kit offers a tractable alternative to mass spectrometry when surveying large sample cohorts. Investigators commonly pair the immunoassay with western blot confirmation when probing mTOR pathway activity, endosome maturation defects, or autophagy flux under pharmacological challenge. Standard plate reader absorbance measurement provides the quantitative readout after the recommended incubation steps.