Human vWF-A2 PharmaGenie ELISA Kit (SBRS1236)

The A2 domain of von Willebrand Factor harbors the Tyr1605-Met1606 cleavage site recognized by ADAMTS13, a metalloprotease whose activity controls VWF multimer length and platelet capture under shear. Quantifying this domain in patient specimens or conditioned media supports researchers investigating thrombotic thrombocytopenic purpura, type 2A von Willebrand disease, and endothelial activation triggered by inflammation or mechanical stress.

Built on the Assay Genie PharmaGenie platform, the kit ships with a pre-coated microplate, matched capture and detection antibodies, recombinant human vWF-A2 calibrators, streptavidin-HRP working solution, TMB substrate, stop reagent, and concentrated wash buffer, along with a step-by-step protocol. Compatible specimens include cell culture supernatants, citrated plasma, and serum prepared by standard procedures, and the absorbance-based readout fits any standard benchtop plate reader.

Routine applications span longitudinal biomarker studies, evaluation of recombinant ADAMTS13 candidates, and characterization of shear-dependent VWF unfolding in flow-chamber or microfluidic models. Components are intended for refrigerated storage upon receipt and the kit is supplied for research use only, not for diagnostic procedures.