Human VPS53 ELISA Kit (HUFI05694)

The Human VPS53 ELISA Kit from Assay Genie enables quantitative measurement of VPS53 protein in human research samples. VPS53 is a core subunit of the GARP (Golgi-associated retrograde protein) complex, which tethers endosome-derived vesicles to the trans-Golgi network and supports retrograde membrane trafficking. Loss-of-function mutations in VPS53 are linked to pontocerebellar hypoplasia type 2E and progressive neurodegeneration, making this target a focus in studies of cerebellar development and trafficking disorders.

This immunoassay is supplied as a complete 96 Assays kit with pre-coated microplate wells, detection antibodies, standards, and buffer reagents. Researchers typically require 100 uL of sample per well, and the assay is calibrated against recombinant human VPS53. Unopened kits should be refrigerated at 4°C, and the reconstituted standard should be aliquoted and frozen at -20°C to limit freeze-thaw cycles. For research use only.