Human VPS4B ELISA Kit (HUFI05692)

Built for researchers studying membrane trafficking and the ESCRT pathway, this Assay Genie kit provides a quantitative immunoassay for human VPS4B, the AAA+ ATPase that drives endosomal sorting, multivesicular body biogenesis, and the abscission step of cytokinesis. The 96 Assays format supports mid-throughput projects in cell biology, viral egress, autophagy, and oncology laboratories where VPS4B expression levels are being investigated in relation to ESCRT-dependent processes.

Adapted for human specimens, the absorbance-based plate workflow delivers reproducible measurements across experimental conditions, time-course experiments, and screening panels. VPS4B is increasingly studied in the context of HIV-1 budding and other enveloped viruses that hijack the ESCRT machinery, as well as in tumor biology where altered vacuolar sorting activity has been linked to proliferation and metastatic behaviour. By providing a reliable readout of VPS4B abundance, the kit enables hypothesis-driven research into these pathways, complementing western blot, qPCR, and microscopy-based approaches.

Each kit from Assay Genie includes all reagents required to complete the assay, and the protocol accommodates standard laboratory equipment found in immunology and biochemistry core facilities. Researchers are advised to review the product insert for detailed preparation, incubation times, and validation data specific to the matrix of interest.